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TTD Cherie : Thank you for contacting the Charter Communications Support Team. My name is Cherie. How may I assist you today? DK: I canceled my phone line with charter about week ago. It should have been disconnected last Monday (06/29/09). Yesterday I received a bill which includes a prorated charge until August. - The phone appears to be still active, at least it is ringing. TTD Cherie : I'm sorry for the inconvenience. I'll be more than happy to assist you with your billing concern. To ensure the security of this account, may I please have the name on the account, complete service address, and phone number? DK: DK DK: 123 My Street DK: City DK: 123 456 7890 TTD Cherie : Thank you. May I please know who I am chatting with? DK: DK TTD Cherie : Thank you for that information. Please give me a moment to pull up your account. TTD Cherie : Thank you for waiting. In order for me to access your account, may I please have the Security Code? It is located at the upper right-hand corner of any bill dated after November 27, 2007. DK: 3969 TTD Cherie : Thank you. I will now set up your PIN because that will be used when requesting for a password reset and account verification in the future. May I please have a four-digit number that you would like to have as a PIN? Please make sure you won't easily forget that. DK: ??? - I want cancel the phone line... TTD Cherie : Yes, but I will need to set up a PIN for your account first. DK: I don't understand. - 1234 TTD Cherie : Thank you. That is now your PIN. Please take note of that as well as your Security Code. TTD Cherie : As I've checked on your account, your phone service was already cancelled. TTD Cherie : Your new balance is $112.40 after the prorated charges. DK: OK. Thanks. - Can you give me some kind of reference number for this chat? TTD Cherie : I am sorry, but we don't have that. You don't have to worry because I will note here in your account that you contacted us today regarding your phone service status inquiry and I provided you with the information you needed. Current Mood: confused
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What do you think is the most annoying thing about Istanbul? 28*C heat with air standing perfectly still and sun burning down your neck sizzling you to a well done condition? Dirt on the marble to the point where you can't make out carvings which are as old as Gondwana? Amount of time your waiter bugged you about most expensive item on the menu? Sorry state of the archaeological monuments which makes one want to weep? Amount of women wearing scarfs and walking behind their husbands? no. no. no. no. no. Men. In-you-face, buy-my-shit, i-don't-take-no-for-an-answer, loud-with-bad-manners, smelly-thinking-they-are-princes-shits-t hat-smell-like-rose-petals, angry-once-you-yell-at-them, horny-son's-of-bitches men. Those men are the most annoying thing in Istanbul. WAY TOO many of them here. Cats are cool. Too bad I hate the bastards (actually, I am allergic to the point of asphyxiation). I have to give them that: Turkish cats have hell of the personality!  Current Mood: annoyed
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Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
Matthew W. Powner1, Béatrice Gerland1 & John D. Sutherland1 1. School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
Correspondence to: John D. Sutherland1 Correspondence and requests for materials should be addressed to J.D.S. (Email: john.sutherland@manchester.ac.uk).
Abstract At some stage in the origin of life, an informational polymer must have arisen by purely chemical means. According to one version of the 'RNA world' hypothesis1, 2, 3 this polymer was RNA, but attempts to provide experimental support for this have failed4, 5. In particular, although there has been some success demonstrating that 'activated' ribonucleotides can polymerize to form RNA6, 7, it is far from obvious how such ribonucleotides could have formed from their constituent parts (ribose and nucleobases). Ribose is difficult to form selectively8, 9, and the addition of nucleobases to ribose is inefficient in the case of purines10 and does not occur at all in the case of the canonical pyrimidines11. Here we show that activated pyrimidine ribonucleotides can be formed in a short sequence that bypasses free ribose and the nucleobases, and instead proceeds through arabinose amino-oxazoline and anhydronucleoside intermediates. The starting materials for the synthesis—cyanamide, cyanoacetylene, glycolaldehyde, glyceraldehyde and inorganic phosphate—are plausible prebiotic feedstock molecules12, 13, 14, 15, and the conditions of the synthesis are consistent with potential early-Earth geochemical models. Although inorganic phosphate is only incorporated into the nucleotides at a late stage of the sequence, its presence from the start is essential as it controls three reactions in the earlier stages by acting as a general acid/base catalyst, a nucleophilic catalyst, a pH buffer and a chemical buffer. For prebiotic reaction sequences, our results highlight the importance of working with mixed chemical systems in which reactants for a particular reaction step can also control other steps.
1. School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
Correspondence to: John D. Sutherland1 Correspondence and requests for materials should be addressed to J.D.S. (Email: john.sutherland@manchester.ac.uk).
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Пациент в Зимбабве: Доктор, у меня отваливаются уши.
Врач в Зимбабве: Да это какая–то xyйня! А что вы делали?
Пациент: E6ал гусей.
Доктор: Так у вас гусиная xyйня!
Пациент: Спасибо, доктор! (умирает)
Доктор (записывает в журнал): Пациент умер от гусиной xyйни.
Пресс–служба минздрава Зимбабве: За прошедшую неделю в Зимбабве умерло две с половиной тысячи человек от голода, пять тысяч четыреста от отравления протухшими бананами и один человек от гусиной xyйни.
Журналист (записывает): Гусиная xyйня вошла в список трех главных причин смертности в Зимбабве.
Новостное агентство: В Зимбабве участились случаи заболевания неизлечимой гусиной xyйней.
Телеканал: Неизвестное ранее заболевание гусиной xyйней выкашивает население Зимбабве. Министерство здравоохранения Зимбабве призывает не паниковать.
Научное светило А: Да, гусиная xyйня не известна науке и в этом ее главная угроза.
Авиакомпания Конго Эйр: Мы прекращаем все полеты в Зимбабве до разрешения эпидемии гусиной xyйни.
Научное светило Б: Власти скрывают! На самом деле гусиная xyйня уже проникла в Европу — в Амстердаме видели чихающего негра с гусем под мышкой.
Пресса (публикует фотографии гусей): Гагакающие убийцы рядом!
Политик: Для борьбы охватившей мир эпидемией гусиной xyйни крайне необходимо истребить всех гусей.
Милиция (козыряет): Будет сделано.
Владельцы гусиных ферм: Да вы что, оxyели? (Красиво дерутся с милицией под щелканье вспышек прессы)
Пресса: Заговор владельцев гусиных ферм угрожает национальной безопасности!
Министр здравоохранения Монголии: Для спасения страны от эпидемии гусиной xyйни, которая вплотную подобралась к нашим границам, нам крайне необходимо выделить сто миллионов долларов на переоснащение лаборатории по борьбе с утиной xyйней.
Премьер–министр Монголии: Да вы оxyели!
Президент Монголии: Премьер–министр слишком погрязла в финансовых махинациях, договорах с Китаем о поставках хлеба и нагло игнорирует реальную угрозу населению свободолюбивой Монголии!
Бывший премьер–министр Монголии: Наша партия срочно требует спасти население от вируса гусиной xyйни. Ну или по крайней мере население восточной Монголии.
Кандидат в президенты Монголии: Единственный способ спасти страну — прекратить отношения с загнивающим западом, откуда пошла гусиная xyйня, а вместо этого уплотнить сотрудничество с Китаем и войти в его состав на правах автономии. Китай нас спасет!
Избиратели Монголии: Да вы оxyели!
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